Transcriptome analysis of lignin-derived aromatics from technical lignins treated with Bacillus licheniformis

Three technical lignins (hydrolysis lignin, HL; kraft lignin, KL; lignosulfonate, LS) were considered for the study. Lignins dissolved in M9-minimal media, along with a glucose (G) control, were batch cultivated with the gram-positive soil bacterium Bacillus licheniformis for 26 hours. Samples were taken in the mid-exponential phase of bacterial growth for transcriptome analysis. The primary goal of this study is to elucidate the genes involved in the utilisation of lignin-derived aromatics. Illumina paired-end sequencing has been performed to address this.

本研究选用三种工业木质素（technical lignin）：水解木质素（hydrolysis lignin, HL）、硫酸盐木质素（kraft lignin, KL）及木质素磺酸盐（lignosulfonate, LS）。将上述木质素溶解于M9基础培养基（M9-minimal media）中，并设置葡萄糖（glucose, G）对照组，与革兰氏阳性土壤细菌地衣芽孢杆菌（Bacillus licheniformis）进行26小时的分批培养。在细菌生长的指数中期阶段采集样本以开展转录组分析。本研究的核心目标是阐明参与木质素衍生芳烃利用的相关基因，并通过Illumina双端测序（Illumina paired-end sequencing）完成相关测序工作以实现该研究目标。