Characterization of the cartilage DNA methylome in knee and hip osteoarthritis.

The aim of this study was to characterise the genome-wide DNA methylation profile of osteoathritis (OA) chondrocytes from both knee and hip cartilage, providing the first comparison of DNA methylation between OA and non-OA hip cartilage, and between OA hip and OA knee cartilage. The study was performed using the Illumina Infinium HumanMethylation450 BeadChip array. Genome-wide methylation was assesed in chondrocyte DNA extracted from 23 OA hip, 73 OA knee and 21 healthy hip controls (NOF - neck of femure samples). Keywords: Methylation profiling by array Bisulphite converted DNA from the 97 samples were hybridised to the Illumina Infinium HumanMethylation450 BeadChip array.

本研究旨在表征膝关节与髋关节软骨来源的骨关节炎（Osteoarthritis, OA）软骨细胞的全基因组DNA甲基化谱，首次实现了OA与非OA髋关节软骨、以及OA髋关节与OA膝关节软骨之间DNA甲基化水平的对比分析。本研究采用Illumina Infinium人类甲基化450K芯片（Illumina Infinium HumanMethylation450 BeadChip array）开展实验。研究对提取自23例OA髋关节样本、73例OA膝关节样本及21例健康髋关节对照（股骨颈（neck of femur, NOF）样本）的软骨细胞DNA进行了全基因组甲基化检测。关键词：基于芯片的甲基化谱分析。将97份样本的亚硫酸氢盐转化DNA（Bisulphite converted DNA）与Illumina Infinium人类甲基化450K芯片进行杂交。