Human breast cancer cells: control vs treated with breast cancer patients' sera

To investigate the significant differently expressed genes upon the stimulation of breast cancer patients' sera. Cells were untreated or treated with 10% breast cancer patients' sera for 24h followed by isolation of total RNA using TRIZOL. Gene-expression profiling was performed on the CapitalBio 35k human Genome Array microchips (Beijing, China).

本研究旨在探究乳腺癌患者血清刺激后出现的显著差异表达基因。实验设置未处理对照组与10%乳腺癌患者血清处理组，将细胞孵育24小时后，采用TRIZOL试剂提取总RNA。随后使用位于中国北京的CapitalBio 35k人类基因组阵列芯片完成基因表达谱分析。