Ovalbumin sensitized and challenged induces differential gene expression in mouse lungs. Mus musculus

To gain insight into the promoting effect of ultrafine particle inhalation on development and progression of allergic asthma, we selected an experimental approach involving exposure to ultrafine carbon particles (UCP) and gene expression profiling of lungs from mice with experimental, ovalbumin induced allergy. Comparative gene expression analysis was performed by hybridizing pooled cDNA samples from lavaged lungs of different groups. These results suggest that allergic sensitization may represent a susceptibility factor for effects of UCP on gene expression in the lung. In sensitized individuals UCP exposure, such as found in polluted air, thus may contribute to the development and /or aggravation of allergic asthma. Keywords: Particle Inhalation, lung, ovalbumin sensitzed and challanged, experssion profiling Overall design: Lungs of groups of six non-sensitized, ovalbumin sensitized, or sensitized and ovalbumin challenged BALB/cJ mice, either subjected to particle-free or UCP containing air; two replicates including one dye swap experiment have been performed for lungs: a) non-sensitized particle free air versus sensitized and ovalbumin challenged sensitized particle free air; b) non-sensitized UCP containing air versus sensitized and ovalbumin challenged sensitized UCP containing air

为探究超细颗粒物吸入对过敏性哮喘发生与进展的促进作用，本研究采用暴露于超细碳颗粒（ultrafine carbon particles, UCP）的实验方案，对卵清蛋白诱导致敏小鼠的肺部组织开展基因表达谱分析（gene expression profiling）。通过对不同组别经支气管肺泡灌洗后的肺部混合cDNA（complementary DNA, cDNA）样本进行杂交实验，完成了对比基因表达分析。
研究结果表明，过敏性致敏或许是肺部基因表达受UCP影响的易感因素。对于已致敏个体而言，暴露于污染空气中常见的UCP，可能会促进过敏性哮喘的发生与/或加重其病情。
关键词：颗粒物吸入、肺部、卵清蛋白致敏及激发、基因表达谱分析
实验设计：将BALB/cJ小鼠分为6组，每组6只，分别为非致敏组、卵清蛋白致敏组、卵清蛋白致敏并激发组，各组小鼠均暴露于无颗粒物空气或含UCP的空气；针对肺部组织完成了2次重复实验，其中包含1次染料互换杂交实验，具体对比组合如下：
a) 非致敏无颗粒物空气组 与 卵清蛋白致敏并激发的无颗粒物空气组；
b) 非致敏含UCP空气组 与 卵清蛋白致敏并激发的含UCP空气组