RNA sequencing data of microglia isolated from brains of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice. {Reporter]

RNA sequencing of FACS sorted microglia isolated from brain tissue of WT, Hexb-tdTomato, Hexb-CreERT2 and Hexb-KO mice was performed to test for potential differences in gene expression caused by the knock-in of tdTomato and CreERT2 into the Hexb locus. Microglia were isolated from brain tissue and RNA expression levels between individual samples were compared.

为探究将tdTomato与CreERT2基因敲入Hexb基因座所引发的基因表达潜在差异，本研究对野生型（Wild Type，WT）、Hexb-tdTomato、Hexb-CreERT2及Hexb-KO小鼠脑组织中分离获得的经荧光激活细胞分选（Fluorescence-Activated Cell Sorting，FACS）分选的小胶质细胞进行了RNA测序。研究人员从脑组织中分离小胶质细胞，并比较了各独立样本间的RNA表达水平。