Alterations in the GyrA Subunit of DNA Gyrase and the ParC Subunit of DNA Topoisomerase IV Associated with Quinolone Resistance in Enterococcus faecalis

The gyrA and parC genes of 31 clinical isolates of Enterococcus faecalis, including fluoroquinolone-resistant isolates, were partially sequenced and analyzed for target alterations. Topoisomerase IV may be a primary target in E. faecalis, but high-level fluoroquinolone resistance was associated with simultaneous alterations in both GyrA and ParC.

本研究对31株粪肠球菌（Enterococcus faecalis）临床分离株（包含氟喹诺酮耐药菌株）的gyrA与parC基因进行了部分测序，并针对其药物靶标突变情况开展分析。结果显示，拓扑异构酶IV（Topoisomerase IV）可能是粪肠球菌的主要氟喹诺酮作用靶标，但高水平氟喹诺酮耐药性与GyrA和ParC同时发生突变存在显著关联。