Comprehensive RNA-Seq profiling in PBMCs of ALS patients and healthy controls

Coding and long non-coding RNA metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from Sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1, C9Orf72 and VCP genes) and healthy controls. Our aim is the whole-transcriptome characterization of PBMCs content, both in terms of coding and non coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Out dataset is a starting point for the omni-comprehensive analysis of coding and long non coding RNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS. whole transcriptome RNA-Seq profiling in PBMCs of ALS and healthy donors

编码RNA与长链非编码RNA的代谢过程，目前已被证实与肌萎缩侧索硬化症（Amyotrophic Lateral Sclerosis, ALS）的发病机制密切相关。本研究针对散发性ALS患者、携带FUS、TARDBP、SOD1、C9Orf72及VCP基因突变的ALS患者，以及健康对照者的外周血单个核细胞（Peripheral Blood Mononuclear Cells, PBMCs）开展了Illumina RNA测序分析。本研究的核心目标为对外周血单个核细胞中的编码RNA与非编码RNA进行全转录组特征解析，以期分别对比散发性ALS患者、突变型ALS患者与健康对照者的转录组表达差异。本数据集可作为基于易获取、易管理、易储存的组织样本开展ALS编码RNA与长链非编码RNA全维度综合分析的研究起点，且该组织样本已被证实为适用于ALS RNA谱分析的理想模型。本数据集包含ALS患者与健康供者外周血单个核细胞的全转录组RNA测序谱数据。