RNA-seq data showing the effects of rpoD mutation t1682c in Pseudomonas fluorescens. RNA-seq data showing the effects of rpoD mutation t1682c in Pseudomonas fluorescens

We report RNA-seq data for four Pseudomonas fluorescens SBW25 types: SBW25 (wild type) and dervied types 6A4, 6B4-Cap- and 6B4-Cap+. The aim of the study is to see the effect of a mutation in rpoD (carried by 6B4) on the transcriptome. Overall design: Method: 3x single colonies of each of SBW25, 6A4 and 6B4 were grown on KB agar, precultured in shaken KB, grown to mid-exponential phase in shaken KB flasks. Total mRNA isolated with RiboPureTM Bacteria Kit (Ambion®). Note that 6B4 was separated into Cap- and Cap+ fractions by centrifugation before RNA extraction. Normalized mRNA-seq library preparation, followed by 100 bp single end sequencing, was performed by the Australian Genome Research Facility (Brisbane, Australia).

本研究报道了4株荧光假单胞菌(Pseudomonas fluorescens) SBW25的RNA测序(RNA-seq)数据，分别为野生型SBW25及其衍生菌株6A4、6B4-Cap-与6B4-Cap+。本研究的目的是探究rpoD基因突变（存在于6B4菌株中）对转录组的影响。
整体实验设计如下：
实验方法：分别对SBW25、6A4与6B4各挑取3个单菌落，先于KB琼脂平板上培养，随后转接至振荡培养的KB液体培养基中进行预培养，最终在振荡培养的KB液体摇瓶中培养至指数生长中期。总mRNA的提取采用RiboPure™ 细菌提取试剂盒（Ambion®公司）完成。需注意：在RNA提取前，通过离心将6B4菌株分为Cap-与Cap+两个组分。标准化的mRNA-seq文库构建及100 bp单端测序工作由澳大利亚基因组研究中心（澳大利亚布里斯班）完成。