In vivo tracking of from the fruiting body of Cordyceps militaris extracts and protective effects against CCl4-induced acute liver injury in mice

Cordyceps militaris is a well-known medicinal and food plant that has attracted academic attention for its polysaccharides with a wide range of physiological activities and health benefits. The active polysaccharide CM-1 was obtained through hot water immersion and column chromatography to separate the pure precip-itate, and its structural characteristics were analyzed using spectral and chemical methods. The antioxidant effect of CM-1 was demonstrated through the DPPH free radical scavenging test and the determination of total antioxidant capacity. Furthermore, iodine substitution absorption utilization was analyzed, and the hepatoprotective effect of CM-1 on CCl4-induced injury in mice was evaluated in vivo. The results showed that CM-1 had an absorption rate of 5.71% in the blood, with the highest content in the liver being 1.24 mg. CM-1 exhibited a positive protective effect on CCl4-induced acute liver injury in mice, reducing serum as-partate aminotransferase (AST) and alanine aminotransferase (ALT) levels, and improving the expression levels of GSH-Px, SOD, and CAT in CCl4-induced liver injury. Additionally, CM-1 treatment helped alleviate the expression of MDA and protected mice from acute liver injury induced by CCl4. These findings suggest that CM-1 has significant potential as a dietary supplement for the prevention and treatment of liver injury.

北冬虫夏草（Cordyceps militaris）是一类广为人知的药食两用资源，其所含多糖因具备多样生理活性与健康益处而受到学界广泛关注。本研究通过热水浸提结合柱色谱法分离纯化得到活性多糖CM-1，并采用光谱学与化学分析法解析其结构特征。通过DPPH自由基清除实验与总抗氧化能力测定，验证了CM-1的抗氧化活性。此外，本研究还分析了CM-1的碘置换吸收利用行为，并在活体水平评估了其对四氯化碳（CCl4）诱导小鼠肝损伤的保护作用。实验结果表明，CM-1在小鼠血液中的吸收率为5.71%，其在肝脏中的最高含量可达1.24 mg。CM-1对四氯化碳诱导的小鼠急性肝损伤具有显著保护作用：可降低血清中天冬氨酸氨基转移酶（AST）与丙氨酸氨基转移酶（ALT）水平，上调四氯化碳肝损伤模型小鼠体内谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）及过氧化氢酶（CAT）的表达水平。此外，CM-1干预可有效降低丙二醛（MDA）的表达水平，进一步减轻四氯化碳诱导的小鼠急性肝损伤。上述研究结果表明，CM-1具备开发为肝损伤防治膳食补充剂的巨大应用潜力。