: Primers for used for quantitative real-time PCR assays.

Primer sequences and positions on the reference sequence are given. Where possible, primers were designed to span an exon-exon junction so as to avoid amplification from potentially contaminating DNA. Amplification efficiencies were calculated from the actual PCR runs as described before.

本数据集已给出参考序列上的引物序列及其对应位置。在条件允许的范围内，引物设计为跨越外显子-外显子接头（exon-exon junction），以避免对潜在污染的基因组DNA进行扩增。扩增效率参照此前已报道的实验方法，通过实际PCR反应计算得到。