Identification of tumor immune evasion mechanism using P0 and P3 cell lines. Mus musculus

The emergence of immune resistance variants during immunotherapy is poorly understood. We generated a highly immune resistant cell line (P3) from a susceptible cell line (P0) by subjecting it to 3 rounds of in vivo immune selection. Subsequently, microarray analysis of P0 and P3 was performed to identify genes that may contribute to the increase in immune resistance. Keywords: cell type comparison Overall design: Four experimental replicates were prepared for each cell line. P0 is the control cell line, and P3 is the experimental/resistant cell line.

免疫治疗（immunotherapy）期间免疫抗性细胞变异株的出现机制目前尚不明确。本研究以易感细胞系（susceptible cell line）P0为起始材料，通过3轮体内免疫筛选（in vivo immune selection），成功构建了高免疫抗性细胞系（highly immune resistant cell line）P3。随后，我们对P0与P3开展了基因芯片分析（microarray analysis），以鉴定可能与免疫抗性增强相关的基因。
关键词：细胞类型比较
实验设计：每个细胞系均设置4个生物学重复。其中P0为对照细胞系，P3为实验抗性细胞系。