Orally administered Odoribacter laneus improves glucose control and inflammatory profile in obese mice by depleting circulating succinate

Total DNA was extracted from frozen human stool, from twenty-five patients with severe obesity, using a commercial DNA extraction kit.Gut microbiota composition was evaluated with metagenomic analysis, using a shotgun sequencing with the Illumina NextSeq with 150 bp x2 chemistry.

本研究采用商用DNA提取试剂盒，从25名重度肥胖患者的冰冻人类粪便样本中提取总DNA。通过宏基因组分析（metagenomic analysis）评估肠道菌群组成，该分析采用搭载150 bp x2化学试剂的Illumina NextSeq测序平台进行鸟枪法测序（shotgun sequencing）。