Transcriptomic Responses of Cannabis sativa L. to Trichoderma hamatum Treated under Drought Conditions

Cannabis sativa L., which has been reclassified as an agronomic crop, has experienced an increase in cultivation. Its interactions with a variety of environmental stressors have been extensively studied. However, the mechanisms of recovery through fungal associations remain underexplored. Trichoderma hamatum, known for its role as a biological agent, enhances plant growth and provides antagonistic defense against pathogenic microbes. This meta-dataset aims to investigate whether Th can enhance drought resistance in a Cannabis plants. Overall design: To investigate how Trichoderma hamatum treatment manifests drought resistance mediation activities at the transcriptome level in Cannabis sativa L., adult Cannabis plants were used. Drought treatment was achieved by halting irrigation, a stress method that hemp can experience in field cultivation. Trichoderma hamatum was isolated from the soil of the cannabis cultivation area using Trichoderma Selective Medium, and then separated into a single fungal strain through the hyphal tip separation process. The fungal strain's TEF1, ITS, and Large Subunit rRNA were sequenced, and identified using NCBI BLASTn. The experimental groups were designed under four different conditions: control, drought stress, T. hamatum single treatment, and a combination of drought stress and T. hamatum treatment.

大麻（Cannabis sativa L.）经重新分类为农艺作物后，其种植规模持续扩大。该物种与多种环境胁迫因子的互作关系已得到广泛研究，但通过真菌共生实现胁迫恢复的机制仍有待深入探索。哈茨木霉（Trichoderma hamatum）作为经典生防菌，可促进植物生长并对病原微生物产生拮抗防御作用。本元数据集旨在探究哈茨木霉是否可提升大麻植株的抗旱能力。
实验整体设计：为探究哈茨木霉处理如何在转录组层面介导大麻的抗旱调控活性，本研究选用成熟大麻植株开展实验。干旱胁迫处理通过停止灌溉实现，该方式可模拟大田栽培中大麻遭遇的自然干旱环境。本研究采用木霉选择性培养基从大麻种植区土壤中分离哈茨木霉，并通过菌丝尖端分离法获得单一真菌菌株；对该菌株的TEF1基因、内转录间隔区（ITS）及大亚基核糖体RNA序列进行测序，通过NCBI BLASTn工具完成菌株鉴定。实验设置四组处理条件：对照组、干旱胁迫组、单施哈茨木霉组，以及干旱胁迫与哈茨木霉联合处理组。