Calibration sausages WGS containing mammalian and avian species

Within this project we extracted and sequenced total DNA of so called calibraton sausages: sausages prepared under laboratory conditions with known compositions of different mammalian and avian species. The data was used to develop and calibrate a new bioinformatic pipeline named AFS (All-Food-Sequencing) that is feasible to accurately determine species composition in food samples in a qualitative and quantitative way by a readmapping/-counting approach.

本项目中，我们对所谓的校准香肠（calibration sausages）的总DNA进行了提取与测序：这类香肠是在实验室条件下制备的，其不同哺乳类与鸟类物种的组成均为已知。所得数据被用于开发并校准一款名为AFS（All-Food-Sequencing）的新型生物信息学流程，该流程可通过读段比对/计数（readmapping/-counting）方法，以定性与定量的方式精准测定食品样本中的物种组成。