Table_3_Comparative transcriptome analysis reveals the molecular mechanism of heat-tolerance in Neopyropia yezoensis induced by Sargassum horneri extract.xlsx

IntroductionHeat stress is one of the main threats for the seaweed aquaculture, leading to the decay of the blades and causing serious economic losses. A newly developed biostimulant, Sargassum horneri extract (SHE), can enhance the heat-tolerance in Neopyropia yezoensis, which can be applied in the seaweed aquaculture. However, the molecular mechanism underlying this acquired heat-tolerance is still unclear.
MethodIn this study, we examined the transcriptional regulation of N. yezoensis under heat stress after the SHE pre-treatment by performing the RNA-Seq on illumina Novaseq platform and differentially expressed gene analysis.
Result and discussionOur results show that, under heat stress, the SHE pre-treatment can alleviate the suppression of genes involved in normal cell activities, such as ribosome biogenesis, photosynthesis, respiration and cell cycle. On the other hand, SHE pre-treatment can also induce some key genes involved in the heat-tolerance. We also found that the combination of SHE pre-treatment and heat stress can induce some genes related to epigenetic modification, suggesting that SHE may facilitate the formation of heat stress memory in N. yezoensis. Our study provides information for understanding the molecular mechanism of heat-tolerance in N. yezoensis and for promoting the application of seaweed-derived biostimulants in the seaweed aquaculture.

引言：热胁迫是海藻养殖面临的主要威胁之一，可导致藻体腐烂并造成严重的经济损失。一种新型生物刺激剂——鼠尾藻提取物（Sargassum horneri extract, SHE）可提升条斑紫菜（Neopyropia yezoensis）的耐热性，该制剂可应用于海藻养殖产业，但该诱导获得的耐热性背后的分子机制仍未明确。
研究方法：本研究通过Illumina NovaSeq平台开展RNA测序（RNA-Seq）并进行差异表达基因分析，探究了鼠尾藻提取物预处理后条斑紫菜在热胁迫条件下的转录调控模式。
结果与讨论：研究结果表明，在热胁迫条件下，鼠尾藻提取物预处理可缓解正常细胞活动相关基因的表达抑制，这些基因涉及核糖体生物发生、光合作用、呼吸作用以及细胞周期等过程。与此同时，该预处理还可诱导部分与耐热性相关的关键基因表达。此外，本研究还发现，鼠尾藻提取物预处理结合热胁迫可诱导表观遗传修饰相关基因的表达，这表明鼠尾藻提取物可能有助于条斑紫菜形成热胁迫记忆。本研究为解析条斑紫菜的耐热分子机制以及推动海藻源生物刺激剂在海藻养殖产业中的应用提供了重要的理论依据。