KINETIC AND COMPOSITIONAL ANALYSIS OF RNA POLYMERASE II IN TRYPANOSOMA BRUCEI

Genome-wide analysis of nascent RNA synthesis gives kinetic information on the transcriptional status of RNA polymerase II (Pol II). In parallel, immunoprecipitation of the largest subunit of Pol II (RPB1) provides the protein composition of the enzyme complex in the presence or absence of the transcriptional inhibitor Actinomycin D.

全基因组新生RNA合成分析可获取RNA聚合酶II（RNA polymerase II，Pol II）的转录状态动力学信息。与此同时，针对Pol II最大亚基（RPB1）的免疫沉淀实验，可获取存在或不存在转录抑制剂放线菌素D（Actinomycin D）时该酶复合物的蛋白质组成。