Protoarray-ATS_110525.xlsx

<em>ProtoArray® screening.</em> The initial screening was performed using ProtoArray® Human Protein Microarrays v.4.0 (Thermo Fisher Scientific, Waltham, MA, USA), which are loaded with 9480 proteins, as described previously (1). A total of 20 serum samples (10 each from HDs and patients with atherosclerosis) were used to select antigenic proteins specifically recognized by serum immunoglobin G (IgG) antibodies. The results were analyzed using Prospector (Thermo Fisher Scientific) software based on M-statistics. When comparing the two groups, a positive cutoff value was calculated for each protein using M-statistics. The percentage of the samples in each group that showed an immune response above the cutoff value was determined and the P-value for the significance of the difference between the two groups was calculated, as previously described (2). Ref. 1. Hamanaka S, Nakagawa T, Hiwasa T, Ohta Y, Kasamatsu S, Ishigami H, Taida T, Okimoto K, Saito K, Maruoka D, et al.: Investigation of novel biomarkers for predicting the clinical course in patients with ulcerative colitis. J Gastroenterol Hepatol 33: 1975-1983, 2018. doi: 10.1111/jgh.14297. PMID:29869393 <br> Ref. 2. Vermeulen N, de Béeck KO, Vermeire S, Van Steen K, Michiels G, Ballet V, Rutgeerts P and Bossuyt X: Identification of a novel autoantigen in inflammatory bowel disease by protein microarray. Inflamm Bowel Dis 17: 1291-1300, 2011. doi: 10.1002/ibd.21508. PMID:21560193

**ProtoArray® 筛选实验**。本研究初始筛选采用ProtoArray®人蛋白质微阵列v4.0（赛默飞世尔科技，美国马萨诸塞州沃尔瑟姆）完成，该芯片共搭载9480种蛋白质，实验流程如既往研究所述(1)。
本研究共纳入20份血清样本，其中10份来自健康供体（Healthy Donors, HD），剩余10份来自动脉粥样硬化患者，旨在筛选可被血清免疫球蛋白G（Immunoglobulin G, IgG）特异性识别的抗原性蛋白质。研究结果基于M统计量，采用Prospector（赛默飞世尔科技）软件进行分析。两组比较时，通过M统计量为每种蛋白质计算阳性临界值；统计两组中免疫反应值高于临界值的样本占比，并计算两组间差异的显著性P值，操作流程如既往研究所述(2)。
参考文献1：Hamanaka S、Nakagawa T、Hiwasa T、Ohta Y、Kasamatsu S、Ishigami H、Taida T、Okimoto K、Saito K、Maruoka D等：溃疡性结肠炎患者临床病程预测新型生物标志物的研究。《胃肠病学和肝病学杂志》，2018，33：1975-1983。DOI: 10.1111/jgh.14297，PMID: 29869393
参考文献2：Vermeulen N、de Béeck KO、Vermeire S、Van Steen K、Michiels G、Ballet V、Rutgeerts P、Bossuyt X：通过蛋白质微阵列鉴定炎症性肠病中的新型自身抗原。《炎症性肠病》，2011，17：1291-1300。DOI: 10.1002/ibd.21508，PMID: 21560193