LncRNA expression profiling of hepatocellular carcinoma cell line BEL-7402 treated with baicalein

Baicalein, a purified flavonoid compound with defined chemical structure extracted from the dry roots of Scutellaria radix that is a broadly used herb in China, has been proved to possess significant anti-hepatoma activity by inhibiting cell proliferation, invasion, metastasis and inducing apoptosis, autophagy via several cancer-related signaling molecules. In recent years, emerging studies have demonstrated that Chinese medicinal herbs can exert their anti-tumor effects through targeting lncRNAs，such as curcumin, camptothecin, resveratrol，paclitaxel. However, there is still no related report about the effects of baicalein on lncRNA expression profile when it exerts its inhibition on hepatoma tumor growth. To investigate whether the antitumor effect of baicalein is related to its modulation of lncRNA expression in hepatocellular carcinoma (HCC), we profiled the lncRNA expression in HCC cell line Bel-7402 treated with baicalein (0, 40, 80μM) for 24 hours using lncRNA microarray and detected the changes by comparing the lncRNA expression profile of HCC cell line Bel-7402 treated with baicalein (40 and 80 μM) and its DMSO control (0 μM). These findings suggest that modulation of lncRNA expression may be an important mechanism underlying the anti-hepatoma effects of baicalein and lay the groundwork for further investigations. To investigate whether the antitumor effect of baicalein is related to its modulation of lncRNA expression in hepatocellular carcinoma (HCC), we profiled the miRNA expression in HCC cell line Bel-7402 treated with baicalein (0, 40, 80μM) for 24 hours using miRNA microarray and detected the changes by comparing the lncRNA expression profile of HCC cell line Bel-7402 treated with baicalein (40 and 80 μM) and its DMSO control (0 μM). These findings suggest that modulation of lncRNA expression may be an important mechanism underlying the anti-hepatoma effects of baicalein and lay the groundwork for further investigations. In this study, we profiled the lncRNA expression in hepatocellular carcinoma cell line Bel-7402 treated with baicalein (40 μM), baicalein (80 μM) and and DMSO control (0 μM) for 24 hours using miRNA microarray, and each of three groups have three biological replicates.

黄芩素（Baicalein）是从中国传统常用中药材黄芩（Scutellaria radix）干燥根中提取的纯化黄酮类化合物，其化学结构明确。现有研究证实，该化合物可通过调控多种癌症相关信号分子，抑制肿瘤细胞增殖、侵袭与转移，并诱导细胞凋亡及自噬，从而发挥显著的抗肝癌活性。
近年来，诸多研究表明，中药可通过靶向长链非编码RNA（long non-coding RNA, lncRNA）发挥抗肿瘤作用，例如姜黄素、喜树碱、白藜芦醇、紫杉醇等。然而，目前尚无关于黄芩素在抑制肝癌细胞生长过程中对lncRNA表达谱产生影响的相关报道。
为探究黄芩素的抗肿瘤作用是否与其调控肝细胞癌（hepatocellular carcinoma, HCC）中lncRNA的表达相关，本研究采用lncRNA芯片（lncRNA microarray）技术，对经0、40、80μM黄芩素处理24小时的HCC细胞系Bel-7402进行lncRNA表达谱分析，并通过对比40、80μM黄芩素处理组与二甲基亚砜（dimethyl sulfoxide, DMSO）对照组（0μM）的lncRNA表达谱，检测其表达变化。本研究结果提示，调控lncRNA表达可能是黄芩素发挥抗肝癌作用的重要机制之一，可为后续相关研究奠定基础。
为探究黄芩素的抗肿瘤作用是否与其调控肝细胞癌（HCC）中lncRNA的表达相关，本研究采用miRNA芯片（miRNA microarray）技术，对经0、40、80μM黄芩素处理24小时的HCC细胞系Bel-7402进行miRNA表达谱分析，并通过对比40、80μM黄芩素处理组与DMSO对照组（0μM）的lncRNA表达谱，检测其表达变化。本研究结果提示，调控lncRNA表达可能是黄芩素发挥抗肝癌作用的重要机制之一，可为后续相关研究奠定基础。
本研究采用miRNA芯片（miRNA microarray）技术，对经40μM黄芩素、80μM黄芩素及DMSO对照组（0μM）处理24小时的肝细胞癌HCC细胞系Bel-7402进行lncRNA表达谱分析，且三组各设置3次生物学重复。