Evaluation of AmpliSeq and SureSelect whole exome sequencing methods on Ion Proton. Homo sapiens

Library preparation for whole exome sequencing is a critical step serving the enrichment of the regions of interest. For Ion Proton, there are only two exome library preparation methods available, AmpliSeq and SureSelect. Here, we evaluated the performance of two libraries using well characterised NA12878 DNA

全外显子组测序（whole exome sequencing）的文库制备是实现目标区域富集的关键步骤。针对Ion Proton测序平台，目前仅可使用两种外显子组文库制备方法：AmpliSeq与SureSelect。本研究采用经过充分表征的NA12878 DNA样本，对这两种文库的性能进行了评估。