Elucidating the molecular mechanisms for CAT-SKL drug effects on breast cancer cells

Time-course analysis was done to measure antioxidant CAT-SKL drug treatment effects on whole genome expression in a breast cancer cell line. total RNA was isolated from parallel cell cultures, starting at the 0 hour (immediately before 1uM CAT-SKL treatment) and every 6 hours after treatment up to 30 hours The MDA-MB-468 breast cancer cell line model was used in this study of logitudinal whole genome mRNA expression data. Expression data were collected by microarray analysis using Illumina HT12v3 arrays.

本研究采用时间进程分析策略，以评估抗氧化剂CAT-SKL（CAT-SKL）药物处理对乳腺癌细胞系全基因组表达的调控效应。实验设置平行细胞培养体系，分别于0小时（即1μM CAT-SKL处理前即刻）、处理后每6小时取样一次，直至30小时，收集样本并提取总RNA。本研究以MDA-MB-468乳腺癌细胞系为模型，开展全基因组mRNA表达的纵向数据分析。表达谱数据通过Illumina HT12v3芯片的微阵列分析完成采集。