CGH: Transgenic soybeans and controls. Glycine max

Transgenic soybean lines were compared to their parental lines by CGH to catalog structurally variant (e.g. deletion and duplication) regions among these genotypes. The CGH comparisons reveal reveal putative deletions and duplications among the 5 lines. Overall design: Transgenic soybean lines were compared to their parent lines ('Bert-MN-01' or 'Wm82-ISU-01', depending on the transgenic plant). Each transgenic line was represented by a single individual T1 generation plant. The reported values show the log2 ratio of the normalized transgenic line hybridization signals (Cy3) over the normalized parent line hybridization signal (Cy5).

本研究通过比较基因组杂交（Comparative Genomic Hybridization, CGH）技术，将转基因大豆株系与其亲本株系进行比对，以归类鉴定各基因型间的结构变异（如缺失与重复）区域。本次CGH比对在5个株系中检测到疑似存在的缺失与重复变异。实验设计概述：将转基因大豆株系与其对应亲本株系（根据转基因事件不同，分别为'Bert-MN-01'或'Wm82-ISU-01'）进行比对；每个转基因株系均以单株T1世代植株作为实验材料。本数据集所报告的数值为经标准化处理的转基因株系杂交信号（Cy3）与亲本株系杂交信号（Cy5）的比值的以2为底的对数值（log₂比值）。