Expression profile of third instar larval fat body and midgut tissues

We use mRNA-seq to transcriptionally profile larval fat body and midgut tissues from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Fat bodies from wandering third instar larvae were dissected from ~50 male larvae and gonads were removed to eliminate contaminating transctips from the gonads. Larval midguts were dissected from ~50 wandering third instar larvae. Larval tissues were removed to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 5ug of total RNA and subject to Illumina based sequencing.

本研究采用mRNA测序（mRNA-seq）技术，对果蝇（Drosophila）三龄幼虫的脂肪体与中肠组织开展转录组谱分析。该数据集可为组织生理学研究提供参考，并可用于鉴定组织特异性转录本。本研究从约50只雄性三龄游走幼虫中解剖获取脂肪体，并去除生殖腺以排除生殖腺来源的转录本污染。同时从约50只三龄游走幼虫中解剖获取幼虫中肠组织。将获取的幼虫组织置于冰上的格雷斯未补充培养基（Graces unsupplemented medium）中，随后采用TRIzol试剂完成RNA提取。从5微克总RNA中制备mRNA-seq文库，并采用Illumina测序平台完成测序。