Spruce dehydrins analysis by LC-MS/MS

Dehydrins (DHNs) are plant-specific proteins that accumulate during the abiotic stress that cause cellular dehydration, such as drought, salinity and freezing. How the dehydrin system in conifers reacts to more short-term drought stress is much less studied. Therefore, we studied the dynamics of dehydrin system in seedlings of Scots pine and Norway spruce both on mRNA and on protein levels under conditions of polyethylene glycole 6000 (PEG)-induced water deficit of different intensities.

脱水素（Dehydrins, DHNs）是一类植物特异性蛋白，会在干旱、盐胁迫与冰冻胁迫等引发细胞脱水的非生物胁迫条件下大量积累。目前针对针叶树脱水素系统如何响应短期干旱胁迫的研究尚且不足。因此，本研究以欧洲赤松和挪威云杉的幼苗为材料，在不同强度聚乙二醇6000（PEG 6000）诱导的水分胁迫环境下，从mRNA与蛋白两个水平探究了其脱水素系统的动态变化。