2016 - eDNA Metabarcoding Inventory of Pacific Northwest Watersheds

Researchers at the Pacific Research Station, USDA Forest Service (USFS) partnered with the Aquatic and Riparian Effectiveness Monitoring Program (AREMP) using multigene eDNA metabarcoding to inventory the biodiversity of 150 watersheds in the Pacific Northwest: including streams in Washington, Oregon and Northern California from 2016, 2017, 2018, 2019 and 2021. Sites are in small streams on USFS and Bureau of Land Management land that are monitored as part of the Northwest Forest Plan. On the same sequence run, there was a smaller project focused on 14 streams on the Olympic Experimental State Forest with samples collected by Washington Department of Natural Resources. This bioproject contains the DNA sequences that will serve as the raw data source for subsequent publications. This work was funded mainly by the USFS, but some support also came from NCASI. This submission contains the reads for the eDNA samples collected in 2016.

美国农业部林务局（USDA Forest Service, USFS）太平洋研究站的研究人员与水生与河岸生态效益监测项目（Aquatic and Riparian Effectiveness Monitoring Program, AREMP）合作，采用多基因环境DNA（eDNA）宏条形码（metabarcoding）技术，对太平洋西北地区的150个集水区开展生物多样性清查，涵盖2016、2017、2018、2019及2021年华盛顿州、俄勒冈州与北加利福尼亚州的溪流。监测点位均设置于隶属于美国林务局与土地管理局（Bureau of Land Management）的小型溪流中，作为《西北森林计划》的常规监测内容之一。在同一批测序流程中，另有一项小型研究项目聚焦奥林匹克州实验林场的14条溪流，其样本由华盛顿州自然资源部采集。本生物项目所包含的DNA序列将作为后续学术出版物的原始数据源。本研究主要由美国林务局资助，同时也获得了NCASI的部分支持。本次提交的数据包含2016年采集的环境DNA样本的测序读段（reads）。