The Xiaogu San Attenuates Pain and Cartilage Damage in Rats with Monosodium Iodoacetate Induced Osteoarthritis

Methods: A total of 24 Sprague Dawley rats were evenly and randomly divided into three separate groups including the normal control (NC), OA and XGS groups. MIA (50 μL, 10 mg/mL) was injected into the left knee joints of the rats to induce OA. After 7 days, The rats of XGS group were given XGS (0.45 g) that was externally applied to the left knee joint, were fixed with gauze and continuously administered XGS for 28 days. Morphological changes in tissues and organs were examined using H&E staining. Biochemical indicators were measured using an automatic biochemical analyzer. Inflammatory cytokines were detected using ELISA kits and immunohistochemistry. RNA-based high-throughput sequencing (RNA-seq) was performed to detect differential expression of mRNAs in normal and MIA–induced OA rats. Detection of gene differential expression in cartilage tissues of normal mice and the monosodium iodoacetate induced osteoarthritis rat.

实验方法：共计24只斯普拉格-道利（Sprague Dawley）大鼠，采用完全随机分组法均分为3组，分别为正常对照组（normal control, NC）、骨关节炎模型组（OA）与XGS干预组。向大鼠左侧膝关节腔内注射单碘乙酸钠（monosodium iodoacetate, MIA）溶液50 μL（浓度为10 mg/mL）以构建骨关节炎模型。造模7天后，XGS干预组大鼠予以0.45 g XGS，将其外敷于左侧膝关节并用纱布固定，持续给药28天。采用苏木精-伊红（Hematoxylin-Eosin, H&E）染色法观察各组大鼠组织与器官的形态学变化；借助全自动生化分析仪检测相关生化指标；采用酶联免疫吸附测定（enzyme-linked immunosorbent assay, ELISA）试剂盒与免疫组化法检测炎症因子水平。基于RNA高通量测序（RNA-seq）技术，检测正常大鼠与MIA诱导的OA模型大鼠关节软骨组织中mRNA的差异表达情况；同时检测正常小鼠与MIA诱导的骨关节炎大鼠软骨组织的基因差异表达水平。