Dataset on particle size of Bacillus subtilis and Chromobacterium violaceum cultivated in different growth medium

Particle size is an important parameter
for assessing the “health status” of microbial cells. For example, cells
deficient in the cell division machinery may be concatenated into long
filaments. This dataset describes some initial observations of number-average
particle size of Bacillus subtilis NRS-762 (ATCC 8473) and genetically
engineered and wildtype Chromobacterium violaceum ATCC 12472 cultivated
in different growth media over different cultivation periods. At appropriate
timepoint, samples were pipetted from the culture broth into 15 mL polypropylene
centrifuge tube. Dilution was applied when necessary to profile for any
dilution related effect on particle size distribution. Vigorous shaking by hand
was used to disperse any cell clusters prior to particle size analysis, which
was carried out with the pipetting of sample into a square cuvette for analysis
in Malvern Zetasizer Nano ZS instrument.

粒径是评估微生物细胞“健康状态”的重要参数。例如，缺乏细胞分裂机制的细胞可能会相互连接形成长丝状结构。本数据集记录了枯草芽孢杆菌（Bacillus subtilis）NRS-762（ATCC 8473），以及基因工程改造株与野生型紫色色杆菌（Chromobacterium violaceum）ATCC 12472，在不同培养基中经不同培养周期培养后的数均粒径初步观测结果。在合适的时间点，使用移液枪从培养液中取样至15 mL聚丙烯离心管内；必要时将进行稀释操作，以分析稀释对粒径分布的相关影响。在粒径分析前，需通过手动剧烈振荡以分散细胞团块；分析时将样品移液至方形比色皿中，使用马尔文粒径电位分析仪（Malvern Zetasizer Nano ZS）完成检测。