Comparative Analysis of microRNA Expression in Serum and Plasma in Patients Screened for BRCA1 or BRCA2 Mutations - dataset 2

This dataset contains serum miRNA expression of 94 healthy women, among which 56 harbored germline BRCA1 or BRCA2 mutations. MicroRNA abundance was quantified with sequencing and qPCR, the latter being the subject of separate GEO submission. Data was analyzed with the aim of assessing concordance between two miRNA assays and the possibility of translating miRNA biomarkers from sequencing to qPCR panel. Generation of this dataset was supported by The Gray Foundation grant “Circulating microRNAs for assessment of risk beyond the BRCA genes and early detection of breast cancer in high-risk families” awarded to Dipanjan Chowdhury and Polish National Research Center grant OPUS “Predictive Potential of Circulating MicroRNA Biomarkers in Patients with High Familial or Genetic Risk of Cancer” (2023/49/B/NZ5/03835) awarded to Wojciech Fendler. Overall design: The experiment was performed in 94 samples of human serum. All samples came from healthy women, but 56 of them were carriers of germinal BRCA1/2 mutations as this experiment is a part of a larger study about changes in miRnome induced by BRCA mutations or the presence of BRCA-related cancers (especially ovarian and breast cancer). In this technical comparison between miRNA quantification methods we included only cancer-free individuals.

本数据集收录94名健康女性的血清微小核糖核酸（microRNA, miRNA）表达数据，其中56人携带生殖系BRCA1或BRCA2突变。研究人员通过测序与定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）对微小核糖核酸的丰度进行定量，其中qPCR检测的相关数据已单独提交至基因表达综合数据库（Gene Expression Omnibus, GEO）。本数据集的分析工作旨在评估两种微小核糖核酸检测方法的一致性，以及将基于测序的微小核糖核酸生物标志物转化为qPCR检测组合的可行性。

本数据集的构建得到两项科研基金的支持：一是授予Dipanjan Chowdhury的格雷基金会项目「循环微小核糖核酸用于评估BRCA基因以外的癌症风险及高危家族乳腺癌早筛」，二是授予Wojciech Fendler的波兰国家研究中心OPUS项目「家族性或遗传性高癌症风险患者循环微小核糖核酸生物标志物的预测潜力」（项目编号：2023/49/B/NZ5/03835）。

实验设计：本实验共纳入94份人血清样本，所有样本均来自健康女性，其中56人为生殖系BRCA1/2突变携带者。本实验为一项更大规模研究的子课题，该研究旨在探究BRCA突变或BRCA相关癌症（尤其是卵巢癌与乳腺癌）诱导的微小核糖核酸组变化。本次微小核糖核酸定量方法的技术对比实验，仅纳入无癌症病史的受试者。