A deregulated HOX gene axis confers an epigenetic vulnerability in KRAS mutant lung cancers [inhibitors]. A deregulated HOX gene axis confers an epigenetic vulnerability in KRAS mutant lung cancers [inhibitors]

MEK and BRD4 inhibitors induce cell death of subset of KRAS-mutant lung tumors in vitro and in vivo We performed microarray analysis of MEK and BRD4 inhibition alone and in combination 24 hours after treatment, prior to the induction of cell death, to analyze transcriptional changes that might be mechanistic drivers of the therapeutic effect Overall design: 12 samples, in triplicate, 3X vehicle, 3X PD-0325901, 3X JQ1, 3X PD-0325901 + JQ1

MEK与BRD4抑制剂可在体外及体内诱导部分KRAS突变型肺肿瘤细胞死亡。本研究于细胞死亡诱导前，即给药后24小时，对单独及联合应用MEK与BRD4抑制剂的样本进行微阵列分析，以解析可能作为治疗效应机制驱动因子的转录组改变。实验整体设计：共12份样本，设置三次生物学重复，其中3份为溶剂对照组、3份为PD-0325901处理组、3份为JQ1处理组、3份为PD-0325901与JQ1联合处理组。