File S1 - Widespread Divergence of the CEACAM/PSG Genes in Vertebrates and Humans Suggests Sensitivity to Selection

The supporting information file contains a total of six supplemental figures (Figures S1–S6) and six supplemental tables (Tables S1–S6). Figure S1. Alignment of CEACAM homologs. Figure S2. Identification of the immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic domain of zebrafish CEACAMs. Figure S3. Schematic representation of a putative evolutionary trajectory of CEACAM genes in teleosts and tetrapods. Figure S4. Schematic representation of CNVs found at the CEACAM/PSG locus based on studies using low-density probes (International HapMap Project webserver). Figure S5. Distribution of allele frequencies of select nonsynonymous SNPs of PSG4, CEACAM1, CEACAM18, and CEACAM21 in HGDP-CEPH world populations. Figure S6. SNPs in CEACAM7-5-6, PSG11-2-5-4, and CEACAM21-4 regions are highly linked in select human populations. Table S1. Inventory of CEACAM family genes in human, chimpanzee, Rhesus monkey, bushbaby, and mouse lemur. Table S2. Inventory of CEACAM family genes in the opossum and the platypus. Table S3. Inventory of CEACAM family genes in nonmammalian vertebrates including, X. tropicalis, D. rerio, G. aculeatus, T. nigroviridis, and T. rubripes. Table S4. PCR primers for the amplification of select CEACAM transcripts in tissues of the platypus, T. nigroviridis, and D. rerio. Table S5. PSG-locus CNVs that were identified based on high-density probes [51], [52], [53]. Table S6. A large proportion of human CEACAM/PSG genes contain SNPs with high population differentiation. (PDF)

本辅助信息文件共计包含6幅补充图（图S1~S6）与6张补充表（表S1~S6）。
图S1：癌胚抗原相关细胞黏附分子（CEACAM）同源序列比对
图S2：斑马鱼CEACAM胞质结构域中免疫受体酪氨酸激活基序（ITAM）的鉴定
图S3：硬骨鱼与四足动物CEACAM基因推定进化轨迹的示意图
图S4：基于低密度探针研究（国际人类基因组单体型图计划（International HapMap Project））服务器所得CEACAM/胎盘特异性糖蛋白（PSG）基因座拷贝数变异（CNV）的示意图
图S5：人类基因组多样性计划-CEPH细胞库（HGDP-CEPH）全球人群中PSG4、CEACAM1、CEACAM18与CEACAM21部分非同义单核苷酸多态性（SNP）的等位基因频率分布
图S6：在特定人群中，CEACAM7-5-6、PSG11-2-5-4与CEACAM21-4区域的单核苷酸多态性（SNP）呈现高度连锁
表S1：人、黑猩猩、恒河猴、婴猴与鼠狐猴CEACAM家族基因目录
表S2：负鼠与鸭嘴兽CEACAM家族基因目录
表S3：非哺乳类脊椎动物CEACAM家族基因目录，涵盖热带爪蟾、斑马鱼、三刺鱼、暗纹东方鲀与红鳍东方鲀
表S4：鸭嘴兽、暗纹东方鲀与斑马鱼组织中部分CEACAM转录本扩增所用的聚合酶链式反应（PCR）引物
表S5：基于高密度探针鉴定得到的PSG基因座拷贝数变异[51]、[52]、[53]
表S6：多数人类CEACAM/PSG基因携带具有显著群体分化的单核苷酸多态性。
（PDF）