Quantitative proteomics of lipid droplets from goose hierarchical granulosa cells and pre-hierarchical granulosa cells

The lipid metabolism of granulosa cells plays a pivotal role in the development of goose follicles. However, the mechanisms by which lipid droplet-associated proteins regulate lipid droplets and influence cellular lipid metabolism remain poorly understood. To address this, our study systematically identified lipid droplet-associated proteins in goose follicular granulosa cells and analyzed these proteins at five distinct time points, in both pre-hierarchical granulosa cells and hierarchical granulosa cells, thereby obtaining relevant information on lipid droplet-associated proteins.

颗粒细胞（granulosa cells）的脂代谢（lipid metabolism）在鹅卵泡（goose follicles）的发育过程中发挥关键作用。然而，脂滴相关蛋白（lipid droplet-associated proteins）如何调控脂滴（lipid droplets）并影响细胞脂代谢的具体机制，目前仍不甚明晰。为解决这一问题，本研究系统鉴定了鹅卵泡颗粒细胞中的脂滴相关蛋白，并分别在前等级颗粒细胞（pre-hierarchical granulosa cells）与等级颗粒细胞（hierarchical granulosa cells）的五个不同时间点对上述蛋白进行了分析，由此获取了脂滴相关蛋白的相关研究信息。