Supporting data for Ethnopharmacological Strategy for Autoimmune Regulation: Cellular Mechanisms and Key Molecular Targets in Sjögren’s Syndrome

This dataset supports the Master of Philosophy thesis entitled "Ethnopharmacological Strategy for Autoimmune Regulation: Cellular Mechanisms and Key Molecular Targets in Sjögren's Syndrome," submitted by Xiaoyu Xu at The University of Hong Kong in August 2025. The research systematically investigates the immunomodulatory effects and therapeutic potential of the classical traditional Chinese medicine formula Yu Ping Feng (YPF) and its key bioactive constituent Atractylenolide I (AT1) in the context of primary Sjögren's syndrome (SS), an autoimmune disorder characterized by exocrine gland dysfunction and systemic immune dysregulation.The dataset includes experimental results from:* Network pharmacology analyses identifying molecular targets and pathways of YPF and AT1 related to immune regulation in SS;* In vivo validation using an experimental SS mouse model assessing salivary gland function, histopathology, autoantibody levels, and immune cell subset composition;* In vitro assays examining the modulatory effects of AT1 on T follicular helper (Tfh) cell differentiation and function in both mouse and human CD4+ T cells;* Transcriptomic profiling and functional validation of the KLF2 signaling pathway as a key molecular mechanism mediating AT1’s immunoregulatory action;* Clinical pilot data from SS patients treated with YPF demonstrating improved clinical symptoms and immune biomarkers.The data comprehensively covers multi-dimensional immune phenotyping, molecular pathway analysis, and therapeutic evaluation, providing a valuable resource for understanding the mechanisms of traditional Chinese medicine in autoimmune disease modulation. Sensitive or identifiable patient information is excluded to protect privacy, and all experimental procedures were conducted following approved ethical guidelines.

本数据集支持香港大学徐晓宇于2025年8月提交的哲学硕士论文《自身免疫调节的民族药理学策略：干燥综合征的细胞机制与关键分子靶点》。该研究系统探究了经典中药方剂玉屏风散（Yu Ping Feng，YPF）及其关键活性成分白术内酯I（Atractylenolide I，AT1）在原发性干燥综合征（primary Sjögren's syndrome，SS）中的免疫调节作用与治疗潜力——原发性干燥综合征是一类以外分泌腺功能障碍与全身免疫失调为特征的自身免疫性疾病。本数据集包含如下实验结果：
* 网络药理学分析：鉴定出YPF与AT1针对干燥综合征免疫调节相关的分子靶点与通路；
* 体内验证实验：采用实验性干燥综合征小鼠模型，评估唾液腺功能、组织病理学特征、自身抗体水平及免疫细胞亚群组成；
* 体外功能实验：检测AT1对小鼠与人类CD4+ T细胞中滤泡辅助性T（T follicular helper，Tfh）细胞分化与功能的调节作用；
* 转录组学分析与功能验证：确认KLF2信号通路是介导AT1免疫调节作用的关键分子机制；
* 临床预试验数据：来自接受玉屏风散治疗的干燥综合征患者，结果显示其临床症状与免疫生物标志物均得到改善。
本数据集全面覆盖多维度免疫表型分析、分子通路解析与治疗效果评估，为阐明中药调节自身免疫性疾病的内在机制提供了宝贵的研究资源。为保护受试者隐私，数据集未包含任何敏感或可识别的患者信息，所有实验操作均严格遵循经批准的伦理准则开展。