Gene expression analysis of two elite breeding barley lines with distinct tolerance to abiotic stress

To investigate the differences in gene expression during germination time course of two elite breeding barley lines (LP104 and LP106), an Agilent oligo microarray was applied. A total of 5320 and 4271 genes were found to be significantly up- and down-regulated, respectively, in LP104 compared to LP106. The highest number of differentially expressed genes (DEGs) was identified after 24h of germination (2260); stages 0h, 2h, 48h, 96h, 120h and 144h were of a lesser magnitude ranging from 929 to 1169 DEGs, and at 72h only 826 DEGs were identified. Analysis of DEGs according to their biological functions revealed that major enriched functional categories among DEGs included RNA, protein, stress, transport and development. Gene expression in germinating grains of two barley lines was measured at 0-144 hours after imbibition. Three biological replications, eight germination stages.

为探究两个优良育种大麦品系（LP104与LP106）在萌发全过程中的基因表达差异，本研究采用安捷伦（Agilent）寡核苷酸微阵列进行检测。相较于LP106，LP104中分别有5320个和4271个基因呈现显著上调与下调表达。萌发24小时后，差异表达基因（differentially expressed genes, DEGs）的数量达到峰值，共计2260个；其余阶段（0h、2h、48h、96h、120h与144h）的差异表达基因数量处于929至1169的较低区间，而72h阶段仅鉴定出826个差异表达基因。对差异表达基因开展生物学功能富集分析后发现，其主要富集的功能类别涵盖RNA、蛋白质、胁迫、转运及发育相关范畴。本研究对两个大麦品系吸胀后0至144小时内的萌发籽粒基因表达水平进行了检测，实验包含3次生物学重复与8个萌发阶段。