CRE-seq of CREs driven by pluripotency factors in mouse ES cells. Mus musculus

A massively parallel reporter assay, CRE-seq, was conducted in mouse embryonic stem (ES) cells. Synthetic cis-regulatory elements (CREs) comprised of binding sites for pluripotency transcription factors were used in the assay. Transcripts of dsRed were PCR amplified from the end of the transcript in order to sequence the barcode sequence in the UTR.

本研究在小鼠胚胎干（ES）细胞中开展了大规模平行报告基因检测（massively parallel reporter assay）实验，该实验即CRE-seq。实验中使用了由多能性转录因子结合位点构成的合成顺式调控元件（cis-regulatory elements，CREs）。为对非翻译区（untranslated region，UTR）内的条形码序列进行测序，研究人员从转录本末端对dsRed的转录本进行了聚合酶链式反应（PCR）扩增。