Uropathogenic E. coli (HCV) Genome sequencing and assembly. Escherichia coli UTI89 isolate:From pellicle

We observed three different matrix-producing variant colonies of Escherichia coli UTI89 population from the biofilm pellicle, when plated on congo red (CR) agar. One among which was a heterogeneous colony variant (HCV), which displayed red dry and rough (RDAR) morphology in the middle layer of the colony, while the peripheral cells displayed white color on CR agar plate. This implied that centre of the colony produces cellulose and curli and the peripheral cells did not. The other two variants that were found were called as red colony variant (RCV) and white colony variant (WCV). In the study, we performed competition studies between the variants and found that the interactions are context dependent.

本研究在刚果红（CR）琼脂平板上培养时，观察到源自大肠杆菌UTI89（Escherichia coli UTI89）种群生物膜菌膜的三种产基质菌落变异体。其中一种为异质性菌落变异体（HCV），其菌落中层呈现红干粗糙型（RDAR）形态，而平板外周的菌落细胞呈白色。这表明该变异体的菌落中心可合成纤维素与卷曲菌毛，而外周细胞则无法产生此类物质。另外两种发现的变异体分别被命名为红色菌落变异体（RCV）与白色菌落变异体（WCV）。本研究通过各变异体间的竞争实验发现，这些相互作用具有上下文依赖性。