Lieb SDQ4129_Y39G10AR18_N2_MXEMB

modENCODE_submission_2970 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Mixed Embryo; Genotype: wild type; Sex: mixed Male and Hermaphrodite population; NUMBER OF REPLICATES: 2; EXPERIMENTAL FACTORS: Developmental Stage Mixed Embryo; temp (temperature) 20 degree celsius; Antibody SDQ4129 Y39G10AR18 (target is Y39G10AR.18); Strain N2

modENCODE_submission_2970 本提交源自Jason Lieb主持的modENCODE项目。modENCODE项目完整清单可参见http://www.genome.gov/26524648。项目目标：本分析聚焦于调控核小体定位与占据状态、控制基因表达结构域、诱导X染色体沉默、指导有丝分裂分离与基因组复制、调控减数分裂过程中同源染色体配对与重组，以及组织细胞核内染色体定位的各类元件。本次筛选得到126个经策略性优选的靶标，涵盖关键组蛋白修饰、组蛋白变体、RNA聚合酶II同工型、剂量补偿蛋白、着丝粒组分、同源染色体配对促进因子、重组标记物以及核膜组成成分。本研究将整合实验数据与靶标生物学的现有认知，对缺失选定靶标蛋白的突变体及RNA干扰提取物开展ChIP-chip分析，利用染色体外阵列评估候选鉴定序列基序在体内招募靶标的能力，鉴定选定靶标的组织特异性表达模式，并构建转录调控与全染色体功能的整合定量模型。数据使用条款与规范请参见http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物样本来源：菌株：N2；发育阶段：混合胚胎；基因型：野生型；性别：混合雄性与雌雄同体种群；重复次数：2；实验因素：发育阶段（混合胚胎）、温度（20摄氏度）、抗体SDQ4129 Y39G10AR18（靶标为Y39G10AR.18）、菌株N2