Mosquito pathogen status (DP1.10041.001)

A set of up to 1000 individual female mosquitoes per species per site per year are targeted for pathogen testing of arboviruses within the families Bunyaviridae, Alphaviridae, and Flavivirdae. From 2013-2019, top-priority mosquito species sent for pathogen testing included: *Aedes aegypti*, *Aedes albopictus* (tested if >100 collected per site-year), and *Culex tarsalis*, *Culex pipiens*, and *Aedes triseriatus* (tested if >200 collected per site-year). From 2013-2019, other individuals, particularly those identified to the species-level within the genera of *Aedes* and *Culex*, were tested if >200 were collected per site-year. Beginning in 2020, the list of vector species sent for pathogen testing was revised and shortened due to low infection prevalence. A smaller set of vectors that reach the minimum abundance threshold of 200 per site-year are selected for pathogen testing from the following species list: *Culex tarsalis*, *Culex restuans*, *Culex pipiens*, *Culex quinquefasciatus*, *Culex erraticus*, *Culex nigripalpus*, *Culex salinarius*, *Aedes japonicus*, *Aedes triseriatus*, *Culiseta melanura*, *Culiseta morsitans*, *Psorophora columbiae*, *Coquillitidia perturbans*, *Aedes dorsalis*, *Aedes trivittatus*, *Aedes canadensis mathesoni*. Instrumentation:

针对每年、每个采样点的各蚊种，选取至多1000只雌性蚊虫，用于布尼亚病毒科（Bunyaviridae）、甲病毒科（Alphaviridae）以及黄病毒科（Flaviviridae）内虫媒病毒的病原体检测。2013年至2019年期间，优先送检的重点蚊种包括：埃及伊蚊（*Aedes aegypti*）、白纹伊蚊（*Aedes albopictus*，若单采样点年捕获量超过100只则开展检测），以及跗斑库蚊（*Culex tarsalis*）、尖音库蚊（*Culex pipiens*）和三带喙伊蚊（*Aedes triseriatus*，若单采样点年捕获量超过200只则开展检测）。2013年至2019年间，其余蚊虫个体——尤其是鉴定至种级的伊蚊属（*Aedes*）和库蚊属（*Culex*）物种——若单采样点年捕获量超过200只，也会被送检开展病原体检测。2020年起，由于感染率较低，送检媒介蚊种的名录经过修订并予以精简。将从以下蚊种名录中选取满足单采样点年捕获量不低于200只最低丰度阈值的少量媒介蚊种，开展病原体检测：跗斑库蚊（*Culex tarsalis*）、扰库蚊（*Culex restuans*）、尖音库蚊（*Culex pipiens*）、致倦库蚊（*Culex quinquefasciatus*）、迷走库蚊（*Culex erraticus*）、黑须库蚊（*Culex nigripalpus*）、盐生库蚊（*Culex salinarius*）、日本伊蚊（*Aedes japonicus*）、三带喙伊蚊（*Aedes triseriatus*）、黑腹饰蚊（*Culiseta melanura*）、具芒饰蚊（*Culiseta morsitans*）、哥伦比亚扰蚊（*Psorophora columbiae*）、扰动寇氏蚊（*Coquillitidia perturbans*）、背点伊蚊（*Aedes dorsalis*）、三纹伊蚊（*Aedes trivittatus*）、加拿大伊蚊马西森亚种（*Aedes canadensis mathesoni*）。检测设备：