Analysis of variance for regression equations.

Millettia speciosa Champ, a member of the Leguminosae family and the genus Millettia, is a rich source of total flavonoids. Investigating the extraction methods and stability of total flavonoids from Millettia speciosa Champ using ultrasound-assisted extraction is crucial for enhancing its medicinal value and advancing the modernization of traditional Chinese medicine. In this study, the optimal extraction conditions were determined as follows, ethanol volume fraction of 64%, ultrasonic temperature of 60°C, solid-liquid ratio of 1:20 g/mL, ultrasonic time of 29 min, and ultrasonic power of 400 W. Under these conditions, the extract of total flavonoids from Millettia speciosa Champ was 6.485%. The stability of the total flavonoids was evaluated under various conditions. The results indicated that the total flavonoids should be stored in the dark. Metal ions such as K+, Na+, Ca2+, Mg2+, and Al3+ had no significant impact on stability, whereas Cu2+, Zn2+, and Fe3+ caused a notable loss of stability. The total flavonoids remained stable under neutral and weakly acidic conditions but were unstable under strongly acidic and alkaline conditions. Additives such as sodium benzoate, sodium chloride, glucose, and sucrose did not significantly affect stability. In contrast, ascorbic acid and citric acid had minimal effects on stability at low concentrations but significantly impacted stability at higher concentrations.

美丽崖豆藤（Millettia speciosa Champ.）隶属于豆科（Leguminosae）崖豆藤属（Millettia），是总黄酮的优质天然来源。采用超声辅助萃取（ultrasound-assisted extraction）法探究美丽崖豆藤总黄酮的提取工艺及其稳定性，对于提升其药用价值、推动中药现代化进程具有关键意义。本研究确定的最优提取条件如下：乙醇体积分数64%、超声温度60℃、料液比1:20 g/mL、超声时长29 min、超声功率400 W。在此条件下，美丽崖豆藤总黄酮的提取率可达6.485%。本研究还对总黄酮的稳定性开展了多场景评价，结果显示：总黄酮应避光储存；K+、Na+、Ca2+、Mg2+及Al3+等金属离子对其稳定性无显著影响，而Cu2+、Zn2+及Fe3+会显著降低其稳定性；总黄酮在中性及弱酸性环境下可保持稳定，但在强酸性与碱性环境下稳定性较差；苯甲酸钠、氯化钠、葡萄糖及蔗糖等添加剂对其稳定性无显著影响；与之相反，抗坏血酸与柠檬酸在低浓度时对稳定性影响微弱，但在高浓度下会显著改变其稳定性。