Data Sheet 4_Evaluation of the safety and functional effects of recombinant humanized type III collagen in food toxicology.pdf

This study aimed to comprehensively assess the edible safety and in vivo bioactivities of recombinant humanized type III collagen (rhCol III). A systematic toxicological evaluation was conducted, including subchronic toxicity tests, bacterial reverse mutation assays, mammalian erythrocyte micronucleus tests, and chromosome aberration tests. Concurrently, efficacy was evaluated using a zebrafish model to investigate multiple biological endpoints. The safety assessment revealed no toxicologically significant abnormalities, confirming a favorable safety profile for long-term oral consumption. In the zebrafish model, rhCol III significantly inhibited the expression of inflammatory factors, reduced the accumulation of advanced glycation end products (AGEs), alleviated oxidative stress damage, and improved molecular indicators associated with skin barrier function. The results demonstrate that rhCol III possesses multifaceted bioactivities, including anti-inflammatory, anti-glycation, antioxidant, and anti-aging properties. This research provides a robust scientific foundation for the development and application of rhCol III in high-value-added oral nutricosmetic products.

本研究旨在全面评估重组人源化III型胶原蛋白（recombinant humanized type III collagen，rhCol III）的食用安全性与体内生物活性。本研究开展了系统毒理学评价，涵盖亚慢性经口毒性试验、细菌回复突变试验、哺乳动物红细胞微核试验以及染色体畸变试验。与此同时，本研究采用斑马鱼模型开展功效评价，探究多项生物学终点。安全性评价结果未发现具有毒理学意义的异常情况，证实该物质长期经口摄入具有良好的安全性。在斑马鱼模型中，重组人源化III型胶原蛋白可显著抑制炎症因子表达，减少晚期糖基化终末产物（advanced glycation end products，AGEs）的积累，缓解氧化应激损伤，并改善与皮肤屏障功能相关的分子指标。研究结果表明，重组人源化III型胶原蛋白具有多维度生物活性，涵盖抗炎、抗糖基化、抗氧化与抗衰老活性。本研究为重组人源化III型胶原蛋白在高附加值口服营养美容产品中的开发与应用提供了坚实的科学依据。