Multifaceted DNA metabarcoding of bat guano

DNA sequences from multiple different loci recovered as part of
multifaceted DNA metabarcoding (MDM) of guano samples collected from two
bat communities. One community was located in New York, USA, and the other
in Arizona, USA. Data classes recovered from each guano sample (individual
scat) using DNA included bat species, bat sex (multiple zinc-finger gene
primer sets), arthropod diet items (16S and 18S rRNA loci), bat parasites
(16S and 18S rRNA loci), and, depending on bat community, exposure to
Pseudogymnoascus destructans (white-nose syndrom pathogen) or plant diet
(trnH-psbA, 18S rRNA loci).

本数据集涵盖从美国纽约州与亚利桑那州的两个蝙蝠群落采集的蝙蝠粪便样本中，经由多维度DNA元条形码（DNA metabarcoding, MDM）技术获取的多个不同基因座的DNA序列。其中一个蝙蝠群落坐落于美国纽约州，另一个位于美国亚利桑那州。每份蝙蝠粪便样本（即单个蝙蝠粪便）经DNA检测可获得以下数据类别：蝙蝠物种信息、蝙蝠性别信息（采用多组锌指基因引物组完成检测）、节肢动物类食物类群（基于16S与18S rRNA基因座）、蝙蝠寄生虫类群（基于16S与18S rRNA基因座）；此外依据蝙蝠群落的不同，还可额外获取两类信息：一是接触白鼻综合征病原菌（Pseudogymnoascus destructans）的暴露情况，二是植物类食物来源（基于trnH-psbA、18S rRNA基因座）。