The Regulatory Network and Role of the circRNA-miRNA-mRNA ceRNA Network in the Progression and the Immune Response of Wilms Tumor based on RNA-seq

Circular RNA (circRNA), which is a newly discovered non-coding RNA, has been documented to play important roles in miRNA sponges, and the dysregulation of which is involved in cancer development. However, circRNA expression profiles and their role in initiation and progression of Wilms tumor (WT) remain largely unclear at present. Here, we used paired WT samples and high-throughput RNA sequencing to identify differentially expressed circRNAs. we used 4 paired WT samples and high-throughput RNA sequencing to identify differentially expressed circRNAs. A complete circRNA-miRNA-mRNA network was then constructed by miRanda prediction tool. Besides these, we selected a key circRNA, circEYA1, for function validation.

环状RNA（circRNA）是一类新近发现的非编码RNA，已有研究证实其可作为miRNA海绵发挥重要生物学功能，且其表达失调与癌症的发生发展密切相关。然而，目前关于肾母细胞瘤（Wilms tumor, WT）中环状RNA的表达谱及其在肿瘤起始与进展过程中的作用仍不甚明晰。本研究共纳入4对配对肾母细胞瘤样本，结合高通量RNA测序技术筛选鉴定差异表达的环状RNA；随后通过miRanda预测工具构建了完整的circRNA-miRNA-mRNA调控网络。此外，本研究选取关键环状RNA circEYA1开展功能验证实验。