Association between the Polymorphisms in Intercellular Adhesion Molecule-1 and the Risk of Coronary Atherosclerosis: A Case-Controlled Study

Intercellular adhesion molecule-1 (ICAM-1), an important immune adhesion molecule, is related to the atherosclerosis. We explored the association between the polymorphisms of the ICAM-1 gene and coronary atherosclerotic stenosis to determine whether any risk factors correlate with genetic polymorphisms in Chinese patients with coronary atherosclerosis. Using the SNaPshot assay, we examined six SNPs of rs5491, rs281428, rs281432, rs5496, rs5498 and rs281437 in 604 patients diagnosed with coronary atherosclerotic stenosis by angiography and in 468 controls. We found that AG genotype of rs5498 had higher frequency in the coronary atherosclerotic stenosis patients (41.56% to 34.19%, P = 0.017, OR = 1.368,95%CI 1.057–1.770) and that the haplotype Ars5491Crs281428Grs281432 had higher frequency in patients (13.8% to 12.1%, P = 0.048). When analyzing the clinical risk factors for coronary atherosclerosis, we found that the rs5498 locus was associated with the levels of apolipoprotein A (APOA) (P = 0.0002) and triglycerides (TG) (P = 0.002). Furthermore, the levels of triglycerides (TG) were also associated with rs281432 (P = 0.040). Additionally, the TT genotype of rs281437 was associated with a higher level of apolipoprotein A (APOA) (P = 0.039) and apolipoprotein B (APOB) (P = 0.003). Finally, among those with coronary atherosclerosis, we found no differences in the haplotype analysis of polymorphisms of the ICAM-1 gene from individuals with hypertension or those who smoked. According to our results, the ICAM-1 polymorphisms were associated with risk of coronary atherosclerotic stenosis in Chinese individuals.

细胞间黏附分子1（Intercellular adhesion molecule-1, ICAM-1）作为重要的免疫黏附分子，与动脉粥样硬化存在关联。本研究旨在探讨ICAM-1基因多态性与冠状动脉粥样硬化性狭窄的相关性，以明确中国冠状动脉粥样硬化患者中是否存在与基因多态性相关的危险因素。研究采用SNaPshot检测技术，对604例经血管造影确诊为冠状动脉粥样硬化性狭窄的患者及468例对照者的rs5491、rs281428、rs281432、rs5496、rs5498及rs281437这6个单核苷酸多态性（Single Nucleotide Polymorphism, SNPs）位点进行了分型分析。结果显示，rs5498位点的AG基因型在冠状动脉粥样硬化性狭窄患者中的检出频率更高（41.56% vs 34.19%，P=0.017，OR=1.368，95%CI：1.057–1.770）；单倍型Ars5491Crs281428Grs281432在患者群体中的频率亦显著升高（13.8% vs 12.1%，P=0.048）。在分析冠状动脉粥样硬化的临床危险因素时，本研究发现rs5498位点与载脂蛋白A（apolipoprotein A, APOA）水平（P=0.0002）及甘油三酯（triglycerides, TG）水平（P=0.002）显著相关。此外，甘油三酯水平亦与rs281432位点相关（P=0.040）。同时，rs281437位点的TT基因型与更高水平的载脂蛋白A（APOA）（P=0.039）及载脂蛋白B（apolipoprotein B, APOB）（P=0.003）相关。最后，在冠状动脉粥样硬化患者中，未发现ICAM-1基因多态性的单倍型分析结果在合并高血压者与吸烟者之间存在显著差异。综上，本研究结果表明，在中国人群中，ICAM-1基因多态性与冠状动脉粥样硬化性狭窄的发病风险相关。