Mapping genomic loci bound to BRD2 in Calu-3 cells. Mapping genomic loci bound to BRD2 in Calu-3 cells

we performed CUT&RUN to comprehensively map genomic loci bound to BRD2 in Calu-3 cells. We also included H3K4me3 and IgG antibodies as postive and negative controls, repectively Overall design: The CUT&RUN experiment was performed with BRD2 antibody as well as the IgG and H3K4Me control antibodies with 1 million Calu-3 cells.

我们采用染色质靶向切割与释放测序（CUT&RUN）技术，对Calu-3细胞中与溴结构域蛋白2（BRD2）结合的基因组位点开展了全面定位分析。实验同时设置组蛋白H3赖氨酸4三甲基化（H3K4me3）抗体与IgG抗体，分别作为阳性对照与阴性对照。总体实验设计：本实验以1×10^6个Calu-3细胞为材料，分别使用BRD2抗体、IgG抗体及H3K4me3抗体完成CUT&RUN实验。