Parasitological and molecular diagnosis of cutaneous leishmaniasis among indigenous peoples in the state of Roraima, Brazil.

Abstract INTRODUCTION: We diagnose cases of cutaneous leishmaniasis (CL) among indigenous peoples of the state of Roraima, Brazil, and discuss some aspects of its epidemiology. METHODS: Skin imprints, and lesion exudate samples collected on filter paper were examined using parasitological and molecular techniques, respectively. RESULTS: Of 30 indigenous individuals, representing several ethnic groups, with suspected cases of CL, 27 (90%) tested positive for Leishmania spp. by PCR, and 21 (70%) by parasitological microscopy. CONCLUSIONS: Cutaneous leishmaniasis is indistinctly present among indigenous peoples from different regions of the state of Roraima. Individuals from seven of the ten existing ethnic groups in the state tested positive for CL, demonstrating the need for further investigation of the disease among these ethnic groups.

摘要 引言：我们针对巴西罗赖马州原住民人群中的皮肤利什曼病（cutaneous leishmaniasis, CL）病例开展诊断，并探讨该疾病的部分流行病学特征。方法：分别采用寄生虫学技术与分子生物学技术，对滤纸片采集的皮肤印片及皮损渗出液样本进行检测。结果：本研究纳入30例疑似皮肤利什曼病的原住民个体，涵盖多个族群；经聚合酶链反应（polymerase chain reaction, PCR）检测，27例（90%）利什曼原虫属（Leishmania spp.）核酸呈阳性，经寄生虫学镜检则有21例（70%）呈阳性。结论：皮肤利什曼病在罗赖马州不同区域的原住民群体中均有分布，无显著地域差异。该州现有10个原住民族群中，有7个族群的个体曾检出皮肤利什曼病阳性，这表明需针对这些族群进一步开展该疾病的相关调查研究。