Transcriptome analysis of Mycobacterium tuberculosis mutant strain devoid of exopolyphosphatases.. Transcriptome analysis of Mycobacterium tuberculosis mutant strain devoid of exopolyphosphatases.

We report the effects of simultaneous deletion of ppx1 and ppx2 on transcription profiles of M. tuberculosis Overall design: RNA was isolated from mid-log phase culture of parental and double mutant strain. Experiments were repeated twice for each strain. Controal samples were S1 and S13 (S1_R1 & S1_R2; S13_R1 & S13_R2). RNA isolated from mutant strains were S2 and S3 (S2_R1& S2_R2; S3_R1 & S3_R2)

本研究报道了ppx1与ppx2同时缺失对结核分枝杆菌（Mycobacterium tuberculosis）转录组谱的影响。整体实验设计：实验材料取自亲本菌株与双基因缺失突变株的对数中期培养物，每株菌株的实验均重复两次。对照样本为S1与S13组，其中S1组包含S1_R1、S1_R2两个生物学重复样本，S13组包含S13_R1、S13_R2两个生物学重复样本；突变株的RNA样本对应S2与S3组，其中S2组包含S2_R1、S2_R2两个生物学重复样本，S3组包含S3_R1、S3_R2两个生物学重复样本。