Size-fractionated gut fluorescence from mesozooplankton collected aboard R/V Point Sur cruise BCS01 in the northern Gulf of Mexico from 2016-02-10 to 2016-02-12

This dataset contains size-fractionated gut fluorescence from mesozooplankton collected using a bongo net aboard R/V Point Sur cruise BCS01 in the northern Gulf of Mexico from 2016-02-10 to 2016-02-12. The samples were always taken from the left net, which had a 202-micrometer mesh size (if different, the mesh size used is indicated in the data table). Samples were transferred into a bucket immediately after retrieval and carbonated water was added to the sample to stop or avoid further 'net feeding' or regurgitation from individual zooplankton. Typically, each net sample was split into two aliquots using a Folsom Splitter aboard the ship. The 1/2 aliquot separated from the original sample was then split further into two subsamples: one was used to measure mesozooplankton gut fluorescence and the other was processed for the dry weight (reported separately). When samples were too thick, the initial sample was split into smaller aliquots (clearly indicated in the dataset). The corresponding aliquot was then size-fractionated into five classes using sieves with meshes measuring: 0.212, 0.5, 1, 2, and 4.75 mm. Filtered seawater was used during sample split and size fractionation. After fractionation, each sample was poured onto a GF filter (2.5 mm diameter) using a filtering system aboard the ship, rinsed with DI water, wrapped in aluminium foil and quick-frozen immediately after filtering by depositing the sample into liquid nitrogen. Before filtering, each sample was kept in the dark in an ice bath. Samples were processed following Strickland and Parsons (1968) spectrophotometric determination of phaeopigments protocol. These data will be used in combination with other mesozooplankton net data to study zooplankton processes in the river-dominated northern Gulf of Mexico. The cruise documentation was provided for the R/V Point Sur cruise BCS01, led by chief scientist Dr. Christian Briseño-Avena.

本数据集包含2016年2月10日至2月12日期间，在墨西哥湾北部由‘Point Sur’号科考船（R/V Point Sur）BCS01航次通过邦戈网（bongo net）采集的中型浮游动物（mesozooplankton）分级粒径肠道荧光数据。样本均取自左侧网具，其网目尺寸为202微米（若有不同，所用网目尺寸会在数据表中注明）。样本回收后立即转入桶中，并加入碳酸水以阻止或避免个体浮游动物进一步的网食行为（net feeding）或反刍。通常，每个网样在船上使用福尔松分样器（Folsom Splitter）分成两份等分样；从原始样本中分离出的1/2等分样再进一步分为两个子样：一个用于测定中型浮游动物肠道荧光，另一个用于干重分析（单独报告）。若样本浓度过高，初始样本会被分成更小的等分样（数据集中有明确标注）。相应等分样随后通过网目尺寸为0.212、0.5、1、2和4.75毫米的筛网分级为五个粒径类。样本分样和分级过程中使用过滤海水。分级后，每个样本通过船上过滤系统倒入直径2.5毫米的玻璃纤维滤膜（GF filter）上，用去离子水（DI water）冲洗，包裹铝箔，并在过滤后立即放入液氮中快速冷冻。过滤前，每个样本需在冰浴中避光保存。样本处理遵循Strickland和Parsons（1968）提出的褐藻色素分光光度测定方案。这些数据将与其他中型浮游动物网采数据结合，用于研究河流主导的墨西哥湾北部浮游动物过程。BCS01航次的相关文档由首席科学家Christian Briseño-Avena博士提供。