Year-round metabolism data from Lakes Simcoe (Ontario, 2010-2011), Diefenbaker, Blackstrap, and Broderick (Saskatchewan, 2013-2014), Canada.

This year-round limnology dataset is from four dimictic Canadian water bodies: three mesotrophic reservoirs in southern Saskatchewan (SK; Blackstrap, Broderick, Diefenbaker) and one oligo-mesotrophic large lake in southern Ontario (Lake Simcoe). Physical, chemical, and biological parameters were measured during the open-water and ice-covered seasons in 2010–2011 (Lake Simcoe) and the SK reservoirs in 2013–2014. Two stations were sampled on Blackstrap reservoir, one on Broderick reservoir, three on Diefenbaker reservoir, and seventeen on Lake Simcoe. Sampling was conducted from a boat during the open-water season and during winter, we accessed the same stations by snowmobile and sampled through holes in the ice. Epilimnetic or surface water was collected for parameters listed below, and δ18O-O2 (oxygen) stable isotope samples were collected from one to 4 depths per station, depending on water column depth and lake thermal structure that day. Parameters reported included photosynthetically active radiation, vertical attenuation coefficient, mean daily mixed layer irradiance, total phosphorus, total dissolved phosphorus, dissolved reactive phosphorus, total dissolved nitrogen, particulate nitrogen, ammonium, nitrate, chlorophyll a, particulate organic carbon, and phytoplankton biovolumes. Photosynthesis irradiance (P-E) parameters were measured including the light saturation parameter, maximum relative electron transport rate through PSII, and the light limited slope of the P-E curve. We also measured areal net productivity, areal gross productivity, and areal respiration via three different methods including fluorometry via a Water-PAM fluorometer, O2 concentrations and δ18O-O2 values, and light-dark bottle experiments measuring changes in O2 concentrations.

本全年湖沼学（limnology）数据集源自加拿大的四个双季对流（dimictic）水体：三个位于萨斯喀彻温省南部（SK；Blackstrap、Broderick、Diefenbaker）的中营养（mesotrophic）水库，以及一个位于安大略省南部的贫中营养（oligo-mesotrophic）大型湖泊（Simcoe湖）。2010-2011年期间（Simcoe湖）及2013-2014年期间（SK水库），在敞水期和冰封期对物理、化学及生物参数进行了测定。Blackstrap水库设2个采样点，Broderick水库设1个，Diefenbaker水库设3个，Simcoe湖设17个。敞水期通过船只采样；冬季则乘坐雪地摩托抵达同一采样点，通过冰孔进行采样。采集表水层或表层水样用于测定下述参数；δ¹⁸O-O₂（氧）稳定同位素样本则根据当日水柱深度及湖泊热结构，在每个采样点的1至4个深度采集。报告的参数包括光合有效辐射（photosynthetically active radiation）、垂直衰减系数、日均混合层辐照度、总磷、总溶解磷、溶解性活性磷、总溶解氮、颗粒态氮、铵态氮、硝态氮、叶绿素a、颗粒态有机碳及浮游植物生物体积。测定了光合-辐照度（P-E）参数，包括光饱和参数、PSII最大相对电子传递速率及P-E曲线的光限制斜率。此外，通过三种不同方法测定了单位面积净生产力、单位面积总生产力及单位面积呼吸作用：使用Water-PAM荧光仪的荧光法、氧浓度及δ¹⁸O-O₂值测定法、以及通过测定氧浓度变化的黑白瓶实验法。