GNPS Penicillium sp. CIGOM10_CONDITIONS_20210511

As part of our investigations on the chemical diversity of organisms from unexplored marine habitats of Mexico, a series of 29 fungal strains isolated from deep-sea sediments (more than 600 m deep) from the Gulf of Mexico were investigated. The antimicrobial potential of their organic extracts from solid cultures grown under the OSMAC approach was assessed against a panel of ESKAPE bacteria and the yeast C. albicans. Chemical studies on the active scaled-up cultures and some small-scale cultures led to the isolation of benzochromenones from Alternaria sp. CIGOM4, benzodiazepines from P. echinulatum CONTIG4, a cytochalsin from Biatriospora sp. CIGOM2, and an imidazopyridoindole from Penicillium sp. CIGOM10. Molecular network analysis by GNPS combined with manual dereplication showed the enormous potential of these fungi to produce bioactive compounds.

本研究作为墨西哥未勘探海洋生境生物化学多样性考察项目的一部分，对分离自墨西哥湾水深超600米深海沉积物的29株真菌菌株展开了系统研究。采用一菌多产物（OSMAC，One Strain Many Compounds）培养策略制备的固体培养物有机提取物，针对ESKAPE耐药致病菌测试组及白色念珠菌（Candida albicans，C. albicans）进行了抗菌活性评估。通过对活性放大培养物及部分小规模培养物开展化学研究，我们从链格孢属（Alternaria sp.）菌株CIGOM4中分离得到苯并色原酮类化合物，从棘孢青霉（Penicillium echinulatum，P. echinulatum）菌株CONTIG4中分离得到苯二氮䓬类化合物，从双囊孢属（Biatriospora sp.）菌株CIGOM2中分离得到细胞松弛素类（cytochalsin）化合物，从青霉属（Penicillium sp.）菌株CIGOM10中分离得到咪唑并吡啶并吲哚类化合物。结合全球天然产物社交分子网络（GNPS，Global Natural Products Social Molecular Networking）的分子网络分析与手动去重复鉴定，证实了这些真菌在产生活性天然产物方面的巨大潜力。