Human Calu-3 cell transcriptome response to a wild type infectious clone of H7N9 Influenza virus and mutant H7N9 viruses. Homo sapiens

The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in human Calu-3 cells infected with WT A/Anhui/1/2013 (H7N9; 'AH1'), AH - NS1-103F/106M, and AH1 - 691 (ferret adapted virus). Overall design: Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 2 days before infection. Prior to infection, washed cells 2 times with PBS, and then infected with a multiplicity of infection of 1. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 7, 12 and 24 h post-infection

本数据集的研究目的为获取感染野生型（WT）A/Anhui/1/2013（H7N9，简称AH1）、AH-NS1-103F/106M以及AH1-691（雪貂适应毒株）的人类Calu-3细胞中，可用于信使核糖核酸（mRNA）、微小核糖核酸（miRNA）、蛋白质组学、脂质组学、代谢组学及组织病理学分析的样本。实验设计：人类Calu-3细胞于感染前2天以每孔1×10^6个细胞的密度接种于6孔板中；感染前使用磷酸盐缓冲液（phosphate buffered saline, PBS）洗涤细胞2次，随后以感染复数（multiplicity of infection, MOI）为1的剂量进行感染。感染组样本设置5个生物学重复，同时平行设置5个生物学重复的时间匹配模拟感染对照样本；采样时间点为感染后0、7、12及24小时。