SH1409537.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为物种界定、识别、学术交流以及基于DNA的物种假说（DNA-based Species Hypotheses，SH）的相关处理工作提供了统一的方法体系。国际核苷酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，均通过应用一系列动态距离阈值（<0.5% - 3.0%）完成聚类，聚类单元近似达到物种级别。所有物种假说均会被赋予唯一且稳定的数字对象唯一标识符（Digital Object Identifier，DOI）格式的名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作进行验证。在为物种假说匹配分类单元名称及其分类层级（如门、纲、目等）时，将尽可能纳入该假说所包含的全部序列的鉴定结果。针对每一个物种假说，均会选取一条自动或人工指定的序列作为其代表序列。这些代表序列已发布于https://unite.ut.ee/repository.php，可供科研界用于本地序列相似性搜索、新一代测序分析流程等科研场景。随着公共真菌ITS序列的数量持续增长，该系统与配套数据集将自动完成更新。