Gene expression profiling in Osteosarcomas

To explore whether KSHV infection is a risk factor for osteosarcoma development in ethnic Uyghur population. Sarcoma specimens including tumor, adjacent normal tissues and paired blood samples were obtained from several osteosarcoma patients of Xinjiang China. Then, osteosarcoma clinical samples were subjected to analysis for the presence of the KSHV genome and proteins. Gene expression profiles of these osteosarcomas were analyzed to reveal the pathogenesis of KSHV-positive and -negative osteosarcomas. mRNA profiles of Osteosarcomas tumors and adjacent normal tissues were generated by high-throughput sequencing, using Illumina Hiseq.

本研究旨在探讨卡波西肉瘤相关疱疹病毒（KSHV）感染是否为维吾尔族人群骨肉瘤发生的危险因素。研究团队从中国新疆地区的多名骨肉瘤患者处获取了标本，包含肿瘤组织、癌旁正常组织及配对血液样本。随后对上述骨肉瘤临床样本开展检测，以分析KSHV基因组与蛋白的存在情况。同时对这些骨肉瘤样本的基因表达谱进行分析，以阐明KSHV阳性与阴性骨肉瘤的发病机制。本研究采用Illumina HiSeq平台通过高通量测序，获取了骨肉瘤肿瘤组织及癌旁正常组织的mRNA表达谱。